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Friday, April 1, 2011

ABSORPTION SPECTRUM OF ALUMINIUM ALIZARIN COMPLEX


ABSORPTION SPECTRUM of aLUMINIUM ALIZARIN COMPLEX
Aim:
To find out the maximum absorption (λmax) of Aluminum - Alizarin complex.
Principle:
Alizarin (1, 2 dihydroxyanthroquinone) is a tricyclic aromatic diketone with two hydroxyl substituents which reacts with alum to form aluminium alizarin complex. Here covalent and coordination bonds fix the aluminium atom to 2 deprotonated alizarin molecules with the additional adjunction of water molecules.
Chemicals required:
1.      Alum -0.1M
2.      Alizarin – 0.001M
3.      Distilled Water
Apparatus required:
1.      Colorimeter
2.      Test Tubes
3.      Glass cuvettes
Procedure:
1.      A series of three test tubes were labeled as A (Alizarin), B (Alum) and C (Alizarin+ alum).
2.      The 5 ml of 0.001M alizarin was pipetted out in to the tube A and 5ml of 0.1M Alum into the B and 0.001M alizarin +0.1M alum was pipetted out into the tube C and the contents of these tubes were mixed well with the cyclomixer.
3.      The maximum absorbance of these solutions was recorded at different wavelength (430nm, 470nm, 490nm, 520nm, 530nm, 580nm, 610nm, 700nm) using water as a blank.
4.      Three different graphs were plotted from this λmax for alum, alizarin and alum-alizarin complex was calculated.
Result:
The maximum absorbance (λmax) of alum was…………………
The maximum absorbance (λmax) of Alizarin was…………………..
The maximum absorbance (λmax) Alizarin – Alum complex was…………….


S. No
Wavelength
Alum
Alizarin
Complex
1
430



2
470



3
490



4
520



5
550



6
580



7
610



8
700




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