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Tuesday, March 29, 2011

LACTOPHENOL COTTON BLUE STAINING


LACTOPHENOL COTTON BLUE STAINING


AIM:
            To identify the fungi by lacto phenol cotton blue staining

MATERIALS REQUIRED:
 Slide
Inoculation loop
Bunsen burner
Fungal Culture
Cover slip
LPCB stain

PROCEDURE:
  1. Take a clean dry glass slide
  2. A drop of LBCB stain was placed in the centre of the slide
  3. With the help of inoculation loop a small portion of colony was scratched and placed in the drop of stain
  4. Then it was teased into small bits with the help of teasing needle
  5. A cover slip was placed on top and gentle pressure was applied for even spreading
  6. It was then observed under the microscope

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